Coding
fhlA-E363

Part:BBa_K778002

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-22)
Revision as of 19:21, 26 September 2012 by YutaOtsuka (Talk | contribs)

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)

FhlA is the trnascriptional activator of hycAp (Part:BBa_778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). 

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None