Coding
Dsr

Part:BBa_K896002:Design

Designed by: Shih-Hao Chen,Yen-Chen Lo,Ting-Yi Lin   Group: iGEM12_NYMU-Taipei   (2012-09-21)
Revision as of 19:16, 26 September 2012 by Lo6203 (Talk | contribs) (→‎Design Notes)

Dsr (sulfite reductase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1636
    Illegal EcoRI site found at 4648
    Illegal PstI site found at 826
    Illegal PstI site found at 3838
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1636
    Illegal EcoRI site found at 4648
    Illegal PstI site found at 826
    Illegal PstI site found at 3838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1636
    Illegal EcoRI site found at 4648
    Illegal BglII site found at 1091
    Illegal BglII site found at 4103
    Illegal BamHI site found at 3007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1636
    Illegal EcoRI site found at 4648
    Illegal PstI site found at 826
    Illegal PstI site found at 3838
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1636
    Illegal EcoRI site found at 4648
    Illegal PstI site found at 826
    Illegal PstI site found at 3838
    Illegal NgoMIV site found at 1741
    Illegal NgoMIV site found at 4753
    Illegal AgeI site found at 406
    Illegal AgeI site found at 1261
    Illegal AgeI site found at 3418
    Illegal AgeI site found at 4273
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Endogenious EcoRI site are at 1636bp and 4143bp, and PstI is at 121bp.

2. Instead of mutant ctting site, we created a smart pSB1C3 plasmid (MfeI-XbaI -pSB1C3-SbfI-SpeI)for CysI cloning.

3. Taking advantage of MfeI and EcoRI are compatible; SbfI and XbaI are compatible.

4. Vector: MfeI-XbaI -pSB1C3-SbfI-SpeI, cut with MfeI and SbfI

5. Insert: CysI, cut with EcoRI and XbaI

Source

YES

References