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Reporter

Part:BBa_K831017

Designed by: Paola Reyes Caldas   Group: iGEM12_Colombia   (2012-09-26)
Revision as of 03:22, 1 October 2012 by Sylvita1015 (Talk | contribs)

PxpsR-EYFP

Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Characterization

The reporter was tested in the pSB1A2 however the part was ensambled into de pSB1C3 backbone and sent to the iGEM Registry Parts. Here we show the basal activity (in absence of 3 OH-PAME) of the PxpsR-eYFP reporter (Image 3) and the functionality


Image 3. Functionality of the reporter PxpsR-eYFP. Bacterials strains were photographed while using Differential Interference Contrast (DIC) microscopy and Fluorescence microscopy using FITC filter. Bacteria transformed with PxpsR-eYFP construct show basal levels of expression of the reporter while none of the bacteria transformed with the promotorless eYFP show fluorescence. High background levels in the promotorless eYFP are due to the autoflorescence of the LB medium and the high exposition.
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Categories
Parameters
n/aPxpsR-EYFP