Part:BBa_K909007

TetR DNA binding domain containing BamHI site for protein fusions

Truncated version of tetracycline repressor TetR, consisting of 1 – 127 amino acids, containing DNA binding domain. The rest of the protein, alpha 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression (see figure). Fusions of TetR-DBD with a dimerizing protein(e.g. UVR8 (link))restores TetR-DBD DNA binding.

Usage and Biology

We introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 382
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]