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Regulatory

Part:BBa_K823041

Designed by: Julia Bartels   Group: iGEM12_LMU-Munich   (2012-09-10)
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PydfG promoter regulated by ecf41

PydfG is a promotor from B. lincheniformes and is regulated by the alternative σ factor ecf41Bli aa 1-204.

SuicideSwitchgrafik.jpg

Our team used this part in the combination with PspoIVB/PsspK,ecf41Bli aa 1-204 and MazF in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].

σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.

This Platereader measurment is the first and not reproduced to this point! PlatereaderPspoIVBecf41PydfG.jpg

This Platereader assay was done with the Bacillus subtilis strain W168 containing thrC::PspoIVB-ecf41Bli aa 1-204 (through transformation with pSBBs4S-PspoIVB-ecf41Bli aa 1-204) and sacA::PydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-PydfG). That we get a signal shows that this is active together with ecf41Bli aa 1-204.

Fig. 1. Platereader measurement of ECF41 delay


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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