Plasmid
Part:BBa_K747101
Designed by: Lucas Schneider Group: iGEM12_Freiburg (2012-09-26)
pTAL-TF
This part can be used to promote gene expression via a vp64-promotor by inserting a twelfe nucleotide binding Tal-Effector. See Bba_K747001 to BBa_K747095
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 966
Illegal suffix found in sequence at 4039 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 966
Illegal SpeI site found at 4040
Illegal PstI site found at 4054
Illegal NotI site found at 972
Illegal NotI site found at 2146
Illegal NotI site found at 4047 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 966
Illegal BglII site found at 1563
Illegal BglII site found at 2914
Illegal BamHI site found at 2217
Illegal BamHI site found at 2920 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 966
Illegal suffix found in sequence at 4040 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 966
Illegal XbaI site found at 981
Illegal SpeI site found at 4040
Illegal PstI site found at 4054 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |