Coding

Part:BBa_K823044

Designed by: Julia Bartels   Group: iGEM12_LMU-Munich   (2012-09-10)
Revision as of 14:05, 26 September 2012 by JuliaB (Talk | contribs)

MazF a bacterial toxin

MazF is a bacterial toxin from E. coli and degrades mRNA

SuicideSwitchgrafik.jpg

Our team used this part in the combination with PydfG,ecf41Bli aa 1-204,PspoIVB/PsspK in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].

σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None