Reporter

Part:BBa_K786010

Designed by: Leung Wai Tak   Group: iGEM12_Hong_Kong-CUHK   (2012-09-21)
Revision as of 07:57, 26 September 2012 by Rickyleung (Talk | contribs)

Gene expression for sensory rhodopsin I & II (SRI & SRII)

This part contains the otnpF promoter, after the stimulation of light, Sensory Rhodopsins are able to control the the autophosphorylation activity of CheA. While CheA can phosphorylate OmpR and phosphorylated OmpR is able to stimulate transcription from the otnpF promoter (part R0083).Must be used in E.coli deficient in EnvZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 659
    Illegal AgeI site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
output
receiver