Measurement

Part:BBa_K911003:Experience

Designed by: Oliver Meacock   Group: iGEM12_Cambridge   (2012-09-12)
Revision as of 22:00, 25 September 2012 by Olijme (Talk | contribs) (→‎Applications of BBa_K911003)

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Applications of BBa_K911003

This riboswitch came to us from the Yale team in the following construct:

F-riboswitch.png

To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below:

FluorideXgal.jpg

The numbers on the tops of the tubes represent the concentration (in mM) of NaF in each culture. The amount of blue is proportional to the amount of β-galactosidase activity present in the culture, which in turn is regulated by the riboswitch. This qualatative assay demonstrated that our part worked.

To get more quantatative data, we performed a Miller assay.

User Reviews

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