Coding

Part:BBa_K863006:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-18)
Revision as of 22:07, 25 September 2012 by Isahu (Talk | contribs) (→‎References)

ecol laccase from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'

Source

The gene sequence was isolated from E. coli BL21(DE3).

References

Zeng, J., X. Lin, et al. (2011). "Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli." Appl Microbiol Biotechnol 89(6): 1841-1849.