Part:BBa_K934022:Experience

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Applications of BBa_K934022

To characterize Plux-LasI(BBa_K934022), we introduced Plux-LasI(BBa_K934022) with Ptet-LuxR(BBa_S03119) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR(BBa_K934011) and Plas-GFP([https://parts.igem.org/Part:BBa_K649001 BBa_K649001) to E.coli as a “Las reporter cell”.

In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(BBa_K934022) synthesized 3OC12HSL.

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