Part:BBa_K323214:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

[http://2012.igem.org/Team:Slovenia 2012 iGEM team Slovenia ] found out that NicTAL part deposited in the Registry by the Slovenian iGEM2010 team was missing subdomain in DNA-binding domain, so our team added this missing part on N terminal sequence. Apart from adding N terminal sequence, we added HIS tag on N terminal end and NLS on terminal C end of NicTAL12 (Figure 1). This construct was later used for designing TAL-based activator and repressor by adding VP16 and KRAB domain.

Figure 1:Sequence alignment of NicTAL10 and NicTAL12, showing differences between constructs.

Results obtained by testing NicTAL12:KRAB repressor proved that NicTAL12 binds to its binding sites better than NicTAL10:KRAB.

Figure 2: Results of testing NicTAL:KRAB repressor demonstrated better binding ability of NicTAL12.

UNIQ98c23dee7be16ccd-partinfo-00000000-QINU UNIQ98c23dee7be16ccd-partinfo-00000001-QINU