Part:BBa_K782069
10x[TALA+TALB] operator
Introduction
TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.
Construct contain ten binding sites while single binding site combines 18 base pairs specificly recognised by TALA.
Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT Binding sites are constructed in alternating setup. Besides TALA binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC wasn't working properly.
Figure 1: Schematic representation of the construct.
Characterization
Construct was later used for constructing different reporter plasmids to show specific binding of TALA KRAB / VP16. Results:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 149
Illegal NgoMIV site found at 514
Illegal AgeI site found at 12
Illegal AgeI site found at 352
Illegal AgeI site found at 377
Illegal AgeI site found at 717 - 1000COMPATIBLE WITH RFC[1000]
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