Composite

Part:BBa_K917005:Experience

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-20)
Revision as of 16:02, 25 September 2012 by Pandeli (Talk | contribs) (→‎Applications of BBa_K917005)

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Applications of BBa_K917005

Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. Nitroreductase_aerobic_culture.jpg

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UNIQ7b7644b2709eab43-partinfo-00000000-QINU UNIQ7b7644b2709eab43-partinfo-00000001-QINU