Coding

Part:BBa_K784038

Designed by: Rachel Lev   Group: iGEM12_Technion   (2012-09-25)
Revision as of 12:15, 25 September 2012 by Rachellev (Talk | contribs)

T7*(K1F) RNA polymerase

This part has the K1F RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells. This part was recieved from Chris Voight lab (see source). In order to achieve reduced levels of toxicity Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and GTG start codon. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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