Composite

Part:BBa_K784039

Designed by: Rachel Lev   Group: iGEM12_Technion   (2012-09-25)
Revision as of 16:08, 25 September 2012 by Rachellev (Talk | contribs)

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T7*(K1F) RNA polymerase + terminator

This part includes T7*(K1F) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(K1F) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.

Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


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