Composite
Part:BBa_K784039
Designed by: Rachel Lev Group: iGEM12_Technion (2012-09-25)
T7*(K1F) RNA polymerase + terminator
This part includes T7*(K1F) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(K1F) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.
Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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