Composite

Part:BBa_K737034

Designed by: Peiran Zhang and Chunyan Zhang   Group: iGEM12_OUC-China   (2012-09-18)
Revision as of 02:50, 25 September 2012 by Five (Talk | contribs)

galK::GFP generator

Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one. 4-1.PNG

Experimental Data

Zpr1.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 819


[edit]
Categories
Parameters
n/agalK::GFP generator