Generator

Part:BBa_K743014

Designed by: Simon Alamos   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 20:09, 24 September 2012 by Csvidal (Talk | contribs)

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after dusk -> LuxAB luciferase (Photorabdus luminiscens)

This part is designed for the expression of the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner with peak 2 hours after subjective dusk (or hour 14 of circadian time).

When transformed into Synechocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.

This part allows the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates).

Placa lux p.l.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1173


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Parameters
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