Translational_Unit

Part:BBa_K733003:Design

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 17:14, 22 September 2012 by Wyq.hkust (Talk | contribs) (→‎Design Notes)

RBS+ydcD: antitoxin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in B. subtilis is designed, aiming at adding RBS directly to the upstream of ydcD gene through PCR.

Source

We obtain this part from the genomic DNA of Bacillus subtilis 168 via PCR amplification.

Forward primer: 5’-aaaggaggtgtatgttttttgtctg-3’ (Prefix not shown)

Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)

References