Coding
Δ15de

Part:BBa_K925001

Designed by: Veronica Rezelj   Group: iGEM12_St_Andrews   (2012-09-22)
Revision as of 21:27, 24 September 2012 by VeronicaRezelj (Talk | contribs)

Delta 15 desaturase

Short description

Delta-15 desaturase involved in an ω-3 biosynthetic pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part encodes a delta-15 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-15 site in the hydrocarbon chain of linoleic acid (18:2 ; Δ9,12), to give alpha- linoleic acid (18:3 ; Δ9,12,15), an ω -3 polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part in addition to Δ -12 desaturase (BBa_K925000), alpha-linoleic acid is observed in membrane lipids.

Characterisation

Results

Conclusion

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Categories
Parameters
None