Coding
Δ12d

Part:BBa_K925000

Designed by: Veronica Rezelj   Group: iGEM12_St_Andrews   (2012-09-22)
Revision as of 19:20, 24 September 2012 by VeronicaRezelj (Talk | contribs)

Delta-12 desaturase


This part encodes a delta-12 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1, Δ9), for its conversion into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part, the bacterial membrane composition is changed, and linoleic acid is observed. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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