Reporter

Part:BBa_K861061

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 09:18, 22 September 2012 by Tinaa (Talk | contribs)

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Efficacy testing RFP generator of BBa_K861060 (PfadR)

We placed BBa_I13507 downstream of BBa_K861060 (PfadR) in plasmid pSB6A1. M9 medium with oleic acid as sole carbon source was used to test the efficacy of the promoter. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5α in the medium for 24h and using SpectraMax M2 plate reader to see its OD 600 and fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


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