Reporter

Part:BBa_K821001:Design

Designed by: Qinfeng Wu, Bin Jia   Group: iGEM12_Tianjin   (2012-09-17)
Revision as of 06:03, 22 September 2012 by Austinamens (Talk | contribs) (→‎Design Notes)

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RFP Reporter with o-RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 769
    Illegal AgeI site found at 881
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the Shine-Dalgarno sequence from the native RBS B0034 to the orthogonal RBS 1 BBa_K821009.

Source

From BioBrick BBa_J04450

References

Lon M. Chubiz,Christopher V. Rao, Computational design of orthogonal ribosomes, Nucleic acids research, Vol. 36, No. 12, 4038–4046