Signalling
Part:BBa_K750114
Designed by: Sifan Wang,Yizhen Yan,Ruosang Qiu,Youbin Mo Group: iGEM12_XMU-China (2012-09-21)
PtetcIT-PcIGLT: atc->cI->GFP(lva)
This part contains PtetcIT(BBa_K750102) and PcIGLT(BBa_K750103). Atc can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2610
[edit]
Categories
Parameters
control | R0040 R0051 |
device_type | signalling |
protein | cI GFP(lva) |