Device
Part:BBa_K786001
Designed by: Leung Wai Tak Group: iGEM12_Hong_Kong-CUHK (2012-09-21)
Revision as of 10:00, 26 September 2012 by Rickyleung (Talk | contribs)
Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
Sensory Rhodopsin II have been fused with HtrII with the a linker peptide, only the membrane-proximal cytoplasmic domain have been kept, while the cytoplasmic domains have been replaced by that of Tsr. Once the fusion protein was triggered, the histidine kinase (CheA) will be up regulated, leading to longer tumbling period(Negative phototaxis), sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 785
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
Illegal NgoMIV site found at 140
Illegal NgoMIV site found at 398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1139
Illegal SapI site found at 913
Illegal SapI.rc site found at 1919
[edit]
Categories
Parameters
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
device_type | |
function | |
protein |