Part:BBa_K801004:Design

pTUM104 yeast shuttle vector with GAL1 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 5799
  Illegal suffix found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5799
  Illegal NheI site found at 3275
  Illegal SpeI site found at 11
  Illegal PstI site found at 25
  Illegal NotI site found at 18
  Illegal NotI site found at 5805
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5799
  Illegal XhoI site found at 5826
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5799
  Illegal suffix found at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5799
  Illegal XbaI site found at 5814
  Illegal NgoMIV site found at 5832
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1432
  Illegal BsaI.rc site found at 2378
  Illegal SapI site found at 349
  Illegal SapI site found at 2530
  

All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

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Design Notes

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Intellectual property:

Corresponding part author/authors:

Source

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