Composite

Part:BBa_K936020

Designed by: Mattan Hamou   Group: iGEM12_UC_Davis   (2012-09-20)
Revision as of 02:22, 4 October 2012 by Ckdeniston (Talk | contribs)

K206000+B0034+pelB+Cutinase+Polyhistidine Tag

Full Cutinase Construct on psB1A3 backbone. UC_Davis_First_pNPB_Run.png
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.UC_Davis_Third_pNPB_Run.png
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell.
UC_Davis_Last_pNPB_Run.png
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 210
    Illegal AgeI site found at 822
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aK206000+B0034+pelB+Cutinase+Polyhistidine Tag