DNA
RS1

Part:BBa_K743000:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 14:50, 20 September 2012 by Juan Alamos (Talk | contribs) (→‎Source)

Synechocystis PCC6803 neutral recombination site.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.


Source

PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.

References