Regulatory
Part:BBa_K818200:Design
Designed by: Emeraldo Jo Group: iGEM12_Groningen (2012-09-19)
Regulator fnr, upregulated by rotten meat volatiles in B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is from B. subtilis genome.
References
Reents H., Münch R., Dammeyer T., Jahn D., Härtig E. (2005).The Fnr Regulon of Bacillus subtilis. Journal of Bacteriology, 188(3):1103-1112