Signalling
Part:BBa_K750000:Design
Designed by: Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu Group: iGEM12_XMU-China (2012-09-18)
pBADGLT:unstable gfp expression device activated by arabinose
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 800
Design Notes
We use the unstable gfp for our project so the degree of fluorescence can be decreased after it get to a top point. In the cell display part, we can achieve the switch of different numbers.
Source
We built this part by using the biobricks from the dna distribution plates 2012.
References
[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5