RNA
TBY

Part:BBa_K897720

Designed by: Muyuan Chen   Group: iGEM12_SEU_O_China   (2012-09-18)
Revision as of 02:58, 24 September 2012 by Arielinseu (Talk | contribs)

Antisense FtsZ protected by paired termini structure under T7 promoter 

In order to interfere with the cell division, we synthesize the right antisense fragments with gene silencing effects targeting ftsZ in E.coli. A vector with paired termini was constructed to constantly express different asRNAs of ftsZ in E.coli,and this part has been found inhibiting cell division considerbly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli
Parameters
device_typeantisense RNA
functioninhibit cell division
targetFtsZ gene