Coding

Part:BBa_K863006:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-18)
Revision as of 21:48, 25 September 2012 by Isahu (Talk | contribs) (→‎Design Notes)

ecol laccase from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'

Source

E. coli BL21 (DE3)

References