Reporter

Part:BBa_K934025:Experience

Designed by: Toshiki Hashimoto   Group: iGEM12_Tokyo_Tech   (2012-09-17)
Revision as of 00:43, 26 September 2012 by Toshiki (Talk | contribs) (→‎Applications of BBa_K934025)

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Applications of BBa_K934025

Pluxtet assay.png

To characterize the function of the Lux/Tet hybrid promoter, we constructed a part Plux/tet-GFP (BBa_ K934024) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline). In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

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