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Part:BBa_K784016:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 16:02, 21 September 2012 by Hila K R (Talk | contribs) (→‎Design Notes)

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Phage Lambda fragment 2 - tail proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
    Illegal AgeI site found at 2771
    Illegal AgeI site found at 3156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3645
    Illegal SapI.rc site found at 923
    Illegal SapI.rc site found at 2591
    Illegal SapI.rc site found at 5507


Design Notes

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other.

Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1
The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp

References