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Part:BBa_K784015:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 15:58, 16 September 2012 by Sl.ilya (Talk | contribs) (→‎Source)

Phage Lambda fragment 1 - head proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
    Illegal BamHI site found at 5526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
    Illegal AgeI site found at 4751
    Illegal AgeI site found at 4900
    Illegal AgeI site found at 6582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2418
    Illegal SapI.rc site found at 6510


Design Notes

Hila, update this.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1
The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp

References