Composite
pT7_xyIE

Part:BBa_K784001

Designed by: Noa Katz   Group: iGEM12_Technion   (2012-09-13)
Revision as of 14:27, 13 September 2012 by Noa (Talk | contribs)

pT7_RBS_xyIE reporter gene

This part's purpose is to testify to the presence of the T7 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T7 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.
The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 540
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None