Measurement

Part:BBa_K911002:Design

Designed by: Oliver Meacock   Group: iGEM12_Cambridge   (2012-09-12)
Revision as of 22:43, 25 September 2012 by Olijme (Talk | contribs) (→‎References)

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Magnesium sensitive riboswitch (8 codon substitution)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original paper mentioned 8 codon substitution of the downstream LacZ reporter gene with the first 8 codons of the MgtE gene (which is the native gene which this riboswitch controls). Our construct used LacI as the downstream regulated sequence, which requires its N-terminal domain to bind to DNA. Sequences both with and without this substitution have been submitted. This contains the substitution. Please note that to use this version effectively, an assembly method that does not leave scars (such as Gibson assembly) will be required.


Source

Bacillus Genome

References

  • Dann C., Wakeman C., Sieling C. Baker S. Irnov I. and Winkler W., Structure and Mechanism of a Metal-Sensing Regulatory RNA, Cell 130, 878–892