Generator

Part:BBa_K861177

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 06:02, 21 September 2012 by Nanhai (Talk | contribs)

K861162+ K861175

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadD.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 815
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1425
    Illegal AgeI site found at 1537
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None