Reporter

Part:BBa_K861173

Designed by: Kuanwei Sheng, Xian Xia   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 17:18, 21 September 2012 by Shinkwee (Talk | contribs)

mRFP generator controlled by PcstA (glucose-repressible promoter)

Lambda cI is controlled by promoter PcstA, which can be activated by CRP-cAMP complex.Therefore protein lambda cI will be expressed in low glucose concentration.This generator contains a RFP downstream the promoter as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amRFP generator controlled by PcstA (glucose-repressible promoter)