Regulatory
Pcar

Part:BBa_K861171:Experience

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 19:38, 26 September 2012 by Nanhai (Talk | contribs) (→‎Applications of BBa_K861171)

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Applications of BBa_K861171

Methods Design of the promoter Pcar which is activated by glucose 

  Promoter Pcar , glucose biosensor plasmid, is derived from constitutive promoter (BBa_J23119) by adding a CRP binding site upstream the promoter which has several base pairs overlapping with polymerase binding site. The sequence was synthesized with restriction enzyme cutting site for EcoRI and XbaI at the 5' terminal and SpeI at 3' terminal. The sequence of promoter Pcar has cohesive terminus at both ends, so it is very convenient for us to construct the plasmid for functional detection.The sequence of Pcar is as followed:

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