Coding

Part:BBa_K936000

Designed by: Nicholas Csicsery   Group: iGEM12_UC_Davis   (2012-07-17)
Revision as of 00:56, 29 September 2012 by Mhamou (Talk | contribs)

LC Cutinase

This is the LC Cutinase gene used to break PET into terephthalic acid and ethylene glycol. The enzyme Cutinase is a lypolytic/esterolytic enzyme that degrades cutin, which is found in most plant and fungi' cuticles. They enzyme itself is more commonly found in plants and bacteria. Cutinase also can degrades water soluble esters and insoluble triglycerides. The enzyme hydrolyzes these substrates by creating an acyl enzyme intermediate. A metagenomic analysis was done in order to find more novel enzymes such as lipases, esterases, proteases, and cellulases, as well as furthering our knowledge of protein sequence space in the environment. Naturally, compost samples would have enzymes that degrade cell walls and other compounds with potentially useful applications. A novel homolog of cutinase, known as Leaf Compost Cutinase (LC) was found to have a 57.4% genetic similarity to cutinase found in T. Fusca, meriting an experiment to overproduce the protein in E. Coli. The study found that it's applications could be most valuable in the textile industry, among other material fields.

It should be noted that ethylene glycol is moderately toxic to humans and other animals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None