Composite

Part:BBa_K902003:Design

Designed by: Emily Hicks   Group: iGEM12_Calgary   (2012-08-29)
Revision as of 02:25, 4 October 2012 by Lisa.O (Talk | contribs) (→‎References)

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Beta-glucuronidase (uidA) with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The beta-glucuronidase (uidA) gene was amplified from the genome of TOP10 E. coli using polymerase chain reaction.

References

Jefferson RA, Burgess SM, Hirsh D. beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51