Part:BBa_K731400

__This part encodes the BBa_K731600 cysteine desulfhydrase (CysDes) from Treponema denticola downstream of a strong expression IPTG inducible cassette K731300 in the pSB1C3 backbone. When transformed in E. coli strain NEB10b and induced with IPTG this biobrick produces an enzyme converting L-cysteine into hydrogen sulfide, pyruvate and ammonia. This part has been successfully operated and characterized both in psB1C3 and the low copy vector pSB4K4. A sfGFP tagged fusion of this part has been deposited as BBa_K731480 and used to test the protein expression levels upon IPTG induction.

ATTENTION: When using this part special handling and precaution should be taken, as this enzyme produces H2S. Manipulation of cells producing this part should be done under a chemical hood.

This part was cloned by the iGEM Trento 2012 team for the creation of an aerobically engineered pathway for the removal of the black crust disfiguring marble stones. Further information about this part and its characterization can be found in the iGEM Trento 2012 wiki page.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1560
  
• 1000
  COMPATIBLE WITH RFC[1000]