Part:BBa_K741018

plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T

The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1589
  Illegal NheI site found at 1612
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1370
  Illegal BglII site found at 1706
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2500
  

Figure 7. shows similar effects of glucose and IPTG on plac-cI-pRM-anticroT-pcon(0.856)-crogfp. pRM is activated by the protein cI. By adding IPTG, plac is activated, which leads to the production of the protein cI. With the existence of cI, pRM-anticroT starts to work and antirco represses the translation of crogfp. On the contrast, glucose represses the expression of plac, which reduces the protein cI. pRM expresses less and the translation of crogfp is repressed less. Therefore, the unit fluorescence intensity of experimental groups with glucose added is the highest.