Composite
araC-CysEm

Part:BBa_K731030:Design

Designed by: Jason Fontana, Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 21:28, 26 October 2012 by Cridelbianco (Talk | contribs) (→‎References)

Inducible araC-pBAD promoter regulating M256I CysE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

K731201 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency.

Source

K731201, K731010

References

Jones-Mortimer, M.C.(1968b). The control of sulphate reduction in Escherichia coli by O-acetyl-l-serine. Biochem. J. 106, 33P.
Kredich & Tomkins, 1966. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. Biochem. J. 241, 4955-4965P.
Denk, D., and A. Bock. 1987. L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. Gen. Microbiol. 133:515–525.