Plasmid_Backbone
pSBBs1C
Part:BBa_K823023
Designed by: Jara Radeck Group: iGEM12_LMU-Munich (2012-08-21)
pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662.
This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
Reference: [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6084
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6090 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 6084
Illegal BamHI site found at 6066
Illegal XhoI site found at 3574 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 6084
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 6084
Plasmid lacks a suffix.
Illegal XbaI site found at 6099
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1128
Illegal NgoMIV site found at 3538
Illegal NgoMIV site found at 4677 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 4501
[edit]
Categories
Parameters
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/assembly
//plasmidbackbone/operation
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/assembly
//plasmidbackbone/operation
chassis | B. subtilis + E. coli |
copies | |
origin | |
resistance | A(E. coli) C(B. subtilis) |