Composite

Part:BBa_K741017:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-08-19)
Revision as of 18:43, 25 September 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

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plac-RBS-cI-T-pRM-anticro-T-pCon(0.244)-RBS-crogfp-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1589
    Illegal NheI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1370
    Illegal BglII site found at 1706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2500


Design Notes

The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited.

Source

BBa_K741001 BBa_K741008 BBa_K741016

References