Coding

Part:BBa_K823020

Designed by: Simon Kelterborn   Group: iGEM12_LMU-Munich   (2012-08-15)
Revision as of 16:31, 15 August 2012 by Dexios (Talk | contribs)

chloramphenicol acetyltransferase (cat)

This part codes for the chloramphenicol acetyltransferase (cat) used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//cds/selectionmarker/antibioticresistance
//chassis/prokaryote/bsubtilis
Parameters
biologyBacillus subtilis
proteincat
resistanceC