Generator
D0

Part:BBa_K738000:Design

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-07-13)
Revision as of 19:11, 24 September 2012 by Smile (Talk | contribs) (→‎Design Notes)

RNA Scaffold generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.

Source

Dr. Camille J. Delebecque, the author of Organization of Intracellular Reactions with Rationally Designed RNA Assemblies, offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).

References

Delebecque C. J., Lindner, A. B., Silver, P. A. & Aldaye, F. A. Organization of intracellular reactions with rationally designed RNA assemblies. Science http://dx.doi.org/10.1126/science.1206938 (2011).