Composite

Part:BBa_K741015:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-25)
Revision as of 18:40, 25 September 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

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plac-RBS-cI-T-pRM-RBS-lysis-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different concentration of IPTG can activate the promoter plac to produce different amount of protein CI, which can consequently activate the promoter pRM. In this case, different amount of lysis will be expressed to destroy the cell.

Source

BBa_K741001 BBa_K741014

References